From the Division of Transfusion Medicine, University of Cambridge/National Blood Service, Cambridge, United Kingdom.
To assess removal of cytomegalovirus (CMV) by leukocyte depletion (LD) filters, we developed a spiking model of latent virus using peripheral blood mononuclear cells (PBMCs) infected by coculture with CMV-infected human fibroblasts. Infected PBMCs were purified by dual magnetic column selection and then spiked into whole blood units or buffy coat pools prior to LD by filtration. CMV load and fibroblast contamination were assessed using quantitative CMV DNA real-time PCR and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) of mRNA encoding the fibroblast-specific splice variant of prolyl-4-hydroxylase, respectively. After correcting for fibroblast-associated CMV, the mean CMV load was reduced in whole blood by LD from 7.42 x 102 to 1.13 copies per microliter (2.8110log reduction) and from 3.8 x 102 to 4.77 copies per microliter (1.910log reduction) in platelets. These results suggest that LD by filtration reduces viral burden but does not completely remove CMV from blood components. (Blood. 2004;103:1137-1139)
Assessment of tricuspid valve annulus size, shape and function using real-time three-dimensional echocardiography
Stress Doppler echocardiography of the internal thoracic artery – a new non-invasive approach for functional assessment after minimally invasive coronary bypass grafting
Non-invasive assessment of differences between bileaflet and tilting-disc aortic valve prostheses by 3D-Doppler profiles
Combined mediastinal node assessment by lymphadenectomy and intraoperative mediastinoscopy
Projected Valve Area at Normal Flow Rate Improves the Assessment of Stenosis Severity in Patients With Low-Flow, Low-Gradient Aortic Stenosis
Incremental Value of Strain Rate Analysis as an Adjunct to Wall-Motion Scoring for Assessment of Myocardial Viability by Dobutamine Echocardiography
Magnetic Resonance Assessment of the Substrate for Inducible Ventricular Tachycardia in Nonischemic Cardiomyopathy
New Noninvasive Method for Assessment of Left Ventricular Rotation
Experimental Validation of a New Ultrasound Method for the Simultaneous Assessment of Radial and Longitudinal Myocardial Deformation Independent of Insonation Angle
Enhanced Risk Assessment in Asymptomatic Individuals With Exercise Testing and Framingham Risk Scores