Little evidence of donor-derived epithelial cells in early digestive acute graft-versus-host disease
From Institut National de la Santé et de la Recherche Médicale (INSERM) ERM-0220, Service de Pathologie, Laboratoire central d'Hématologie, Service Commun d'Imagerie Moléculaire et Cellulaire, Service d'HépatoGastroentérologie, and Service d'Hématologie/Greffe de Moelle, Institut Universitaire d'Hématologie, Université Paris VII, Hôpital Saint Louis, AP-HP, Paris, France.
Donor origin of epithelial intestinal cells has been studied in animals and humans after transplantation and has been used as evidence of hematopoietic stem cell (HSC) plasticity. However, in the human gastrointestinal tract, no study used X- or Y-chromosome detection by fluorescence in situ hybridization (FISH) coupled with immunologic stainings to characterize cell types on the same tissue section. Here, we combined these techniques on the same section of duodenal epithelium in 6 patients with acute graft-versus-host disease. Donor-derived lymphoid cells were detected in the epithelium and the lamina propria, as expected. However, using our stringent criteria, no donor-derived cells could be proven to be epithelial.
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Acute graft-versus-host disease in patients with Fanconi anemia or acquired aplastic anemia undergoing bone marrow transplantation from HLA-identical sibling donors: risk factors and influence on outc
Adult human hematopoietic cells provide functional hemangioblast activity
Annexin II mediates plasminogen-dependent matrix invasion by human monocytes: enhanced expression by macrophages
Antibody response to DBY minor histocompatibility antigen is induced after allogeneic stem cell transplantation and in healthy female donors
Bcl-xL antagonism of BCR-coupled mitochondrial phospholipase A2 signaling correlates with protection from apoptosis in WEHI-231 B cells
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《血液学杂志》2004年1月第103卷第1期